Figure 3.

Intranuclear and surface staining of human PBMC stimulated for 24 and 72 h with 1 μg well⁻¹ muromonab-CD3, TGN1412, IgG1 and IgG4κ isotype controls in a solid phase assay. CD3+CD4+ T-cells are shown in red. Events in the right hand quadrants are positive for FoxP3 and events in the upper quadrants are positive for CD25. T-cells with the regulatory phenotype CD4+CD25+FoxP3+ are increased following stimulation with muromonab-CD3 (8.4 ± 1.5% and 20.7 ± 1.7% at 24 and 72 h, respectively) and TGN1412 (11.3 ± 1.9% and 15.6 ± 1.6% at 24 and 72 h, respectively), compared with isotype controls. Data from one representative donor are shown. , CD3+ CD4+