Table 2.

Primer sequences for redox and anti-inflammatory target genes analysed in this study

Target	Sense (5′–3′)	Antisense (3′–5′)	Source/reference
GR	TGC CTG CTC TGG GCC ATT	CTC CTC TGA AGA GGT AGG AT	(Cao et al. 2006)
GST-A1	GAA GCC AGT CCT TCA CTA CT	CAG CTC TTC CAC ATG GTA GA	(Cao et al. 2006)
NQO1	CCA TTC TGA AAG GCT GGT TTG	CTA GCT TTG ATC TGG TTG TC	(Cao et al. 2006)
GPx	CTC TCC GCG GTG GCA CAG T	CCA CCA CCG GGT CGG ACA TAC	(Yeh & Yen, 2006)
TrxR2	TGT CAA CGA GCA CAC AGT TCA CGG	ACG TTT TCC CAG GGG ACT CCT TCA	(Yonehara et al. 2003)
TXN	TGC CGA CCT TCC AGT TCT AT	GGC TTC GAG CTT TTC CTT GT	(Khanna et al. 2012)
HO-1	TTT GTC CGA GGC CTT GAA GG	TCA TGC GAG CAC GAT AGA GC	(Tamion et al. 2002)
GCLC	ACC CCG AGC CCA CTC AGA CG	GGG CGG AAA CCT GGT GGC TG	NIH Primer BLAST
GPx4	CGA GAT GAG CTG GGG CCG TC	CCA CAC TCG GCG TAT CGG GC	NIH Primer BLAST
β-Actin	CCC AGC ACA ATG AAG ATC AA	GAT CCA CAC GGA GTA CTT G	(Khanna et al. 2012)

The source of each primer sequence is indicated either from primary literature or as generated in our laboratory using the NIH Primer Blast resource (http://www.ncbi.nlm.nih.gov/tools/primer-blast/). Abbreviations: GCLC, γ-glutamylcysteine ligase catalytic subunit; GPx, glutathione peroxidase; GPx4, glutathione peroxidase 4; GR, glutathione reductase; GST-A1, glutathione s-transferase A-1; HO-1, haem oxygenase-1; NQO1, NAD(P)H:quinone oxidoreductase 1; TXN, thioredoxin; and TxnRd2, thioredoxin reductase-2.