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. 2013 May 13;591(Pt 14):3549–3563. doi: 10.1113/jphysiol.2013.257741

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© 2013 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

© 2013 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Recordings of autofluorescence at 450 nm with 340 nm excitation showing effects of graded levels of hypoxia from 5/10% down to anoxia. A and B, original recordings. C and D, normalised summary data (mean ± SEM) from 9 (C) and 11–17 (D) recordings with best fit rectangular hyperbola. The recording and graph on the left (A and C) were obtained in a normal Tyrode containing 2.5 mm Ca²⁺; the recording and graph on the right (B and D) were obtained in a Ca²⁺-free Tyrode containing 100 μm EGTA. Note rapid effects of hypoxia at all levels.