Figure 3. Effects of graded hypoxia on NADH autofluorescence in SCG neuron.

Recording of autofluorescence at 450 nm with 340 nm excitation showing effects of graded levels of hypoxia from 2.5% down to anoxia. A, original recording from a single neuron conducted in Ca²⁺-free Tyrode containing 100 μm EGTA. B, normalised summary data (mean ± SEM) from 10 neurons with best fit rectangular hyperbola (black line, see Results for details). Also shown in B, in grey, are data and best fit rectangular hyperbola for type-1 cells recorded under identical conditions reproduced from Fig. 2D. Note minimal effects of hypoxia even at 0.5%; only anoxia causes a robust increase in NADH autofluorescence in SCG neurons.