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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Traffic. 2012 Oct 10;13(12):1589–1600. doi: 10.1111/tra.12008

Figure 5. Comparison of diffusion coefficients for EGFP in the cytosol obtained by confocal FRAP under different experimental conditions as calculated using the Soumpasis equation or DConfocal equation.

Figure 5

Diffusion coefficients were determined by FRAP data fitting (DFitting, Eq. 3), by the DConfocal equation (Eq. 5), by the Soumpasis equation using rn (Drn), and by the Soumpasis equation using re (Dre) for individual confocal FRAP curves (N≥6). Dashed box indicates the range of EGFP’s diffusion coefficients in the cytosol reported in the literature. re was measured from an averaged postbleach profile (n=1,N=10 cells) and D’s were obtained from individual FRAP data (n=1, N=8,10,10, and 8 cells). *, p<0.05 compared to DFitting, Student’s t-test.