Table 1.
Analytic 2D FRAP models:
| References | Bleaching Geometry | Laser Profiles | Postbleach Profile | Diffusion during photobleach | Data types | Microscopy | Target proteins |
|---|---|---|---|---|---|---|---|
| Kang et al. (17) | circle in R2 | Gaussian (rn) | Gaussian (re) | corrected | f(t) | CLSM/ wide-field static | Slow/Fast |
| Smisdom et al. (24) | circle in R2 | Gaussian (ρ) | Uniform with Gaussian edge | Not corrected | f(t) | CLSM | Slow |
| Axelrod et al. (10) | circle in R2 | Gaussian (rn)/Uniform (rn) | Gaussian (rn)/Uniform (rn) | Not corrected | f(t) / τ1/2 | wide-field static | Slow |
| Braga et al. (15) | circle in R2 | Uniform (rn) | Gaussian (re) | corrected | f(t) | CLSM | Slow/Fast |
| Soumpasis (11) | circle in R2 | Uniform (rn) | Uniform (rn) | Not corrected | f(t) / τ1/2 | wide-field static | Slow |
| Sprague et al. (25) | circle in R2 | Uniform (rn) | Uniform (rn) | Not corrected | f(t) | wide-field static | Slow/Fast |
| Waharte et al. (26) | circle in R2 | NA | Gaussian(re) | corrected | f(x, t) | CLSM | Slow/Fast |
| Current Study | circle in R2 | Gaussian (rn) | Gaussian (re) | corrected | f(t)/τ1/2 | CLSM/ wide-field static | Slow/Fast |
| Angelides et al.(21) | circle in a box | Gaussian (rn) | Gaussian (rn) | Not corrected | f(t) | wide-field static | Slow |
| Deschount et al. (22) | box in R2 | Gaussian (ρ) | Uniform with Gaussian edge | Not corrected | f(t) | CLSM | Slow |
| Seiffert & Oppermann (23) | circle/line in R2 | NA | Gaussian (re) | corrected | f(x,t)/re(t) | CLSM | Slow |
R2: infinite 2 dimensional plane, rn: bleaching spot radius= static laser radius, ρ: scanning laser radius, re: effective radius measured from a fluorescent intensity profile, f(t): fluorescence intensity from an ROI, f(x,t): time course of spreading radius of postbleach profiles.