Table 2.

List of parameters and their definitions

Parameter	Meaning	Parameter	Meaning
D	Diffusion coefficient	Fi	Prebleach fluorescence intensity
τ1/2	Half time of recovery	F0	Initial postbleach fluorescence intensity
re	Nominal radius = bleaching spot radius =sampling region radius	F∞	Postbleach steady state fluorescence intensity
re	Effective radius = Spreading radius of post-bleach profile	F1/2	Fluorescence intensity at the half of recovery = [F0 + F∞]/2
q	Quantum yield	Mf	Mobile fraction = [F∞ − F0]/[Fi − F0]
ε	Attenuation factor for excitation laser	τD	$\mathrm{Diffusion\; time}=r_e^2/(4D)$
K	Bleaching depth parameter	Drn	D from Soumpasis Eq. using $r_n(D_{r_n}=0.224\frac{r_n^2}{{\tau }_{1/2}})$
γ	The ratio of the nominal and effective radius = rn/re	Dre	D from Soumpasis Eq. using $r_e(D_{r_e}=0.224\frac{r_e^2}{{\tau }_{1/2}})$
Ci	Prebleach fluorescent protein concentration	DConfocal	D from rn, re and ${\tau }_{1/2}(D_{\mathit{confocal}}=\frac{r_n^2+r_e^2}{8{\tau }_{1/2}})$
n	Number of independent experiments	N	Total number of cells for FRAP data collection