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. 2013 Aug 2;3:2346. doi: 10.1038/srep02346

Figure 4. Effect of pretreatment with TPG on the expression of Grp78 (A), Mt1 (B), or Atf4 mRNA (C) in C2C12-DMPK160 cells.

Figure 4

Total RNA was extracted from cells treated with 0.1 or 0.3 μg/ml TPG for 16 h, or cells exposed to 0.4 μM MeHg with or without 100 μM Trolox. Expression of Grp78, Mt1, or Atf4 mRNA was analyzed by quantitative real-time PCR. The histogram depicts the indicated mRNA normalized to β-actin. Values shown are means ± SE of 4 separate experiments. *, **Significantly different from TPG- and MeHg-untreated cells by a one-way ANOVA (*p < 0.05, **p < 0.01). #, ##Significantly different from Trolox-untreated cells by a one-way Welch's t-test (#p < 0.05, ##p < 0.01).