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. 2013 Jul 26;4:2218. doi: 10.1038/ncomms3218

Figure 2. TERT transcript expression according to mutation status of the TERT promoter.

Figure 2

(a) TERT messenger RNA (mRNA) is not expressed in 4 normal livers, 24 cirrhosis and 48 HCAs, whereas it is overexpressed in 156 HCCs mutated and 109 HCCs not mutated for the TERT promoter. (b) TERT transcript was overexpressed in all mutated cirrhotic macronodules (n=4) when compared with non-mutated macronodules (n=8). (c) TERT mRNA was not overexpressed in HCA with CTNNB1 mutations (n=9) compared with HCA without CTNNB1 mutations (n=39). (d) β-catenin target genes (GLUL and LGR5) were not overexpressed in HCC harbouring TERT promoter mutations without CTNNB1 mutations (n=82) compared with HCC lacking both TERT promoter and CTNNB1 mutations (n=80). TERT, GLUL and LGR5 mRNA levels were measured using quantitative RT–PCR. All the results were normalized with the mean of normal liver tissues (see Methods section). Results were reported in median with interquartile range and compared using non-parametric Mann–Whitney test. Differences were considered significant when the P-value was <0.05.