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. Author manuscript; available in PMC: 2013 Aug 2.
Published in final edited form as: Nat Biotechnol. 2012 Jul 1;30(7):701–707. doi: 10.1038/nbt.2288

Figure 3. CTX/TLC Assembly and Validation.

Figure 3

A) Alignment of C2 Data (> 5kb) on the CTX H1 assembly. B) Strobe and C) continuous reads were used to create an initial scaffold of the contigs within the CTX/TLC region. Concordant strobe reads (with spans between 5.5–7kb) are shown over the region. C) Long reads were used to fill-in gaps/resolve tandem repeat structures; selected long reads (> 1.5kb) are shown in the region. D) Ordering and directionality of CDC contigs (colored directed blocks) and genes (small black arrows). Each CDC contig is given a different color to highlight repeated elements. E) PCR primers were designed to validate the region upstream of CTX as well as the TLC structure. F) PCR products were sequenced and mapped back to confirm the structure; a sampling of subreads (> 5kb) that aligned to the products is shown.