Skip to main content
. Author manuscript; available in PMC: 2013 Aug 2.
Published in final edited form as: J Viral Hepat. 2010 Nov;17(11):757–769. doi: 10.1111/j.1365-2893.2009.01231.x

Figure 3. Type I IFN Production of Monocytes and Dendritic Cells Incubated with HCV-JFH.

Figure 3

Monocytes (A and D), immature DC (B and E) and mature DC (C and F) were incubated with HCV and cells were collected at day 1 and day 7 post infection. Total RNA was isolated and IFN-β and IFNα2a mRNA expression was analyzed by real-time RT-PCR against unincubated controls and normalized against the housekeeping gene GAPDH. Monocytes (G) and immature DC (H) were either incubated by HCV alone or co-incubated with Newcastle Disease Virus (NDV). IFNβ was measured by Real Time RT-PCR. Bars represent the standard error (SEM) of three separate experiments (three separate donors).