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. Author manuscript; available in PMC: 2013 Aug 2.
Published in final edited form as: J Viral Hepat. 2010 Nov;17(11):757–769. doi: 10.1111/j.1365-2893.2009.01231.x

Figure 4. Lack of HCV Replication on Dendritic Cells Incubated with HCV-JFH1.

Figure 4

Incubated cells were analyzed for HCV replication by immunofluorescence and by Real Time RT-PCR. A) Relative HCV expression in monocytes, immature DC and LPS matured DC after infection with MOI=0.1 of HCV-JFH1 particles. B) Relative NS5A expression in monocytes, immature DC and LPS matured DC after infection with MOI=0.01 HCV-JFH1 particles. C) Unincubated immature DCs (negative control) were stained with NS5A with FITC conjugated secondary antibody. D) Immature DC incubated with MOI=0.1 HCV. E) Huh 7.5 cells previously transfected with HCV-JFH1 plasmid (virus source, positive control).