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. 2013 Jul 9;3(7):e277. doi: 10.1038/tp.2013.50

Figure 2.

Figure 2

Western blots of candidate antigens. (a) Purified lactate dehydrogenase (LDH, containing A and B subunits) or recombinant full-length Cypin, Y-box-binding protein (YBX1), collapsin response mediator protein (CRMP) 1, CRMP2 and stress-induced phosphoprotein 1 (STIP1) proteins were probed with plasma from mothers of children with autism (AU) or mothers of typically developing (TD) controls diluted to 1:400, P indicates polyclonal antibody positive controls. Arrows indicate location of band(s) of interest. Note the different patterns of reactivity to LDH A and B subunits in lanes 1–3 of the LDH panel. This reactivity is further characterized in panel b where the blot of recombinant human glutathion-S-transferase (GST)-tagged LDHA (A), recombinant human GST-tagged LDHB (B) or purified native human LDH (Enz) was probed with maternal plasma diluted to 1:400, demonstrating variable reactivity to the LDH subunits. Also of note, as demonstrated in Table 1, some mothers of typically developing children have antibodies to the individual proteins and it is reactivity to the specific antigen combinations that confers specificity for MAR autoantibodies.