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Effect of CNN2 and SDK1 silencing on expression of EMT markers and β1-integrin. LNCaP cells were seeded and transfected with non-targeting control (c) siRNAs, or siRNAs directed against CNN2 or SDK1 expression. Four days after transfection, cells were harvested and western blot analysis was performed using antibodies directed against the EMT markers β-catenin, claudin-1, E-cadherin and ZO-1 (A), β1-integrin (B) and β-actin (β-act).
