Fig. 6.

CNN2 and SDK1 regulate organization of the actin cytoskeleton and cell migration in CR-CaP cells. C4-2 cells (A) and LN-Rf (B) cells were seeded on cover slips and transfected with non-targeting control siRNA (left image), or siRNAs targeted against CNN2 (middle image) and SDK1 (right image). After 96 h, cells were fixed and stained with rhodamine phalloidin as described (left panel). Protein extracts from C4-2 and LN-Rf cells that were transfected as above were analyzed via western blotting using antibodies directed against CNN2, SDK1 or β-actin (β-act) (right panel).