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Metabolic lability of sirolimus in recombinant P450 and FMO enzymes. Sirolimus (3 µmol/l) was incubated with recombinant drug-metabolizing enzymes. P450 concentration was set at 50 pmol/ml. Protein concentration of recombinant FMO enzyme was set at 0.5 and 1.0 mg/ml in pH = 9.5 glycine buffer (a) and pH = 7.4 potassium phosphate buffer (b), respectively. Data represent the mean of duplicate determinations.
