FIG. 4.

Wnt5a is a promigratory factor for hESC-CMs. (A) We screened 10 soluble factors for effects on hESC-CM migration in the transwell assay. The only factor showing a significant promigratory effect was Wnt5a, which induced an approximately twofold increase in migration at concentrations of 100 and 500 ng/mL. N=4, *P<0.05. (B) During live-cell microscopy of hESC-CMs on unpatterned FN, Wnt5a accelerated random migration by approximately twofold. N=20, *P<0.05. (C, D) Representative images of hESC-CMs in the gap-closure assay after 24-h exposure to the control-CM (C) or Wnt5a-CM (D). (E) Wnt5a-treated cells migrated to cover an approximately twofold more surface area than control cells, a response that was inhibited by UM206 (100 nM). *P<0.05, N=4. Color images available online at www.liebertpub.com/scd