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. Author manuscript; available in PMC: 2013 Aug 2.
Published in final edited form as: Immunol Invest. 2012;41(0):680–710. doi: 10.3109/08820139.2012.703745

Figure 2.

Figure 2

Phenotypes of human mDSCs. Analysis of peripheral blood mononuclear cells (Ficoll interface) from patient with renal cell carcinoma. Initial gating on myeloid (CD33) cells with absent to low HLA-DR expression (A) reveals three populations: neutrophilic-MDSCs (note, normal neutrophils concentrate in the RBC pellet rather than Ficoll interface) which are CD15posCD14neg (green boxes, B, C, E); monocytoid MDSCs which are CD15negCD14pos (blue boxes, B, C, F) and prelineage forms (B, C G). As in mouse spleen, n-MDSCs predominate in human peripheral blood of patients with RCC (C).