Phenotypes of human mDSCs. Analysis of peripheral blood mononuclear cells (Ficoll interface) from patient with renal cell carcinoma. Initial gating on myeloid (CD33) cells with absent to low HLA-DR expression (A) reveals three populations: neutrophilic-MDSCs (note, normal neutrophils concentrate in the RBC pellet rather than Ficoll interface) which are CD15posCD14neg (green boxes, B, C, E); monocytoid MDSCs which are CD15negCD14pos (blue boxes, B, C, F) and prelineage forms (B, C G). As in mouse spleen, n-MDSCs predominate in human peripheral blood of patients with RCC (C).