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. Author manuscript; available in PMC: 2013 Aug 2.
Published in final edited form as: Arch Biochem Biophys. 2008 Nov 13;481(2):197–201. doi: 10.1016/j.abb.2008.11.008

Figure 1. Increases in human SMIT2 mRNA level, [3H]D-chiro-inositol (DCI), and [3H]myo-inositol uptake upon human SMIT2 cDNA overexpression in L6 myoblasts.

Figure 1

Levels of human SMIT2 mRNA and rat cyclophilin A (panel A) on pooled total RNA isolated from cells transfected with pcDNA3.1 (n=4 wells), pcDNA3.1-EGFP (n=4), or pcDNA3.1-H-SMIT2 (n=4) were determined by quantitative real-time PCR as described in Materials and Methods. Uptake of [3H]DCI (B) and [3H]myo-inositol (C) was assayed from individual wells (n=4 for each transfection) as described in Materials and Methods. The level of statistical differences relative to pcDNA3.1 is indicated by **P < 0.01 (for DCI and myo-inositol uptake).