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. Author manuscript; available in PMC: 2013 Aug 2.
Published in final edited form as: Arch Biochem Biophys. 2008 Nov 13;481(2):197–201. doi: 10.1016/j.abb.2008.11.008

Figure 2. Kinetics of [3H]D-chiro-inositol (DCI) upon human SMIT2 overexpression.

Figure 2

L6 myoblasts transfected with pcDNA3.1-H-SMIT2 were incubated for 1 hour in uptake buffer containing 1.2 μCi/mL tracer and various concentrations of unlabeled DCI, myo-inositol, pinitol, or glucose. No unlabeled competitor was added to cells transfected with pcDNA3.1 only and this uptake (shown by the closed circle) was subtracted as the endogenous uptake from those transfected with pcDNA3.1-H-SMIT2. Displacement of tracer by unlabeled DCI (panel A), myo-inositol (B), pinitol (C), and glucose (D) is shown. Points represent the mean and standard error of the mean (n=4 wells each). Km (for DCI and myo-inositol) or Ki (for pinitol or glucose) and maximum binding were calculated from Eadie-Hofstee plot of the data (plot not shown).