FIG. 4.

AAV2 serine/threonine/lysine mutant vectors demonstrate increased transduction efficiency in vitro. HeLa cells were either mock-infected or infected at 2×10³ viral genome (VG)/cell with AAV2-WT or AAV2 S/T→A (A) or AAV2 K→R (C) mutant vectors and cells were analyzed for EGFP expression 48 hr later by flow cytometry. The percentage of EGFP-positive cells posttransduction with either serine/threonine mutants (B) or lysine mutants (D) is shown. Similar experiments were carried out in HEK-293 cells with AAV2-WT or AAV2 S/T/K mutant vectors at an MOI of 2×10³ VG/cell (E). Quantitative analysis of these data by flow cytometric analysis is shown in (F). The data depicted in (A), (C), and (E) are representative histograms whereas the data in (B), (D), and (F) are means of triplicate analyses. One-way analysis of variance (ANOVA) was used for statistical analysis.*p<0.05, **p<0.01 versus AAV2-WT-infected cells. Color images available online at www.liebertpub.com/hgtb