Table 2.
Physical Particle Packaging Titers (Viral Genomes/ml) of AAV2 Serine/Threonine/Lysine Mutant Vectors
| Serine (S)→Alanine (A)a | Threonine (T)→Alanine (A)a | Lysine (K)→Arginine (R)a |
|---|---|---|
| S276A (1.65×1010) | T251A (1.8×1012) | K39R (2.4×1011) |
| S489A (3.2×1012) | T454A (2.5×1010) | K137R (3×1012) |
| S498A (1×1012) | T503A (5.25×1010) | K143R (2.3×1012) |
| S525A (3.2×1012) | T671A (1.6×1012) | K161R (9×1011) |
| S537A (8×1011) | T701A (3.2×1012) | K490R (2.3×1011) |
| S547A (1.6×1012) | T713A (3.2×1012) | K507R (2×1011) |
| S662A (3.2×1012) | T716A (5.25×1010) | K527R (3.2×1011) |
| S668A (4×1011) | K532R (2.4×1012) | |
| K544R (3×1011) | ||
| K527R+K532R (6×1011) | ||
| K490R+K532R (2×1011) |
a
Average packaging titers from at least two packaging experiments. Vectors were generated by polyethyleneimine-based triple transfection of AAV-293 cells. The vectors were purified by iodixanol gradient ultracentrifugation and column chromatography (mentioned in Materials and Methods) and resuspended in a final volume of 0.5 ml of phosphate-buffered saline. The titers of wild-type self-complementary AAV2 vectors ranged between 4×1011 and 1×1012 VG/ml in the laboratory. VG, viral genomes.