Table 3.

Neutralizing Antibody Titers: AAV2 S→A Vectors Compared with AAV2-WT^a

Serum no.	Group	Reciprocal NAb Titer
1	scAAV2-WT	5,120
2	S489A	640
3	S525A	5,120
4	S537A	5,120
5	S547A	5,120
6	S662A	5,120
7	Anti-AAV2 rabbit control serum	81,920

^aAAV2 S489A vector demonstrates lower neutralization antibody titers compared with the WT-AAV2 vector. Pooled serum samples from WT-AAV2- or AAV2 mutant-injected mice (n=4 per group) were analyzed for neutralizing antibodies 4 weeks after vector administration. Values are the reciprocal of the serum dilution at which relative luminescence units (RLUs) were reduced 50% compared with virus control wells (no test samples).