Table 1.

Nucleotide Sequence of the Mutation Primers Used for Site-Directed Mutagenesis in Adeno-Associated Virus Serotype 8 Capsid, and the Change in Nucleotide and Restriction Patterns of Silent Mutation Obtained with the Primers

Residue	Sequence (5’ to 3’)	Nucleotide change	Change in restriction enzyme due tosilent mutation
S279A	Wild-type primer sequence:	AGC→GCC	SfcI disappears
	CTACTTCGGCTACAGCACCCCCTGGGGG
	Mutant Primer Sequence:
	CTACTTCGGCTACGCCACCCCCTGGGGG
S501A	Wild-type primer sequence:	AGC→GCC	G→T AgeI appears
	GACAACCGGGCAAAACAACAATAGCAACTTTGCCTGGACT
	Mutant primer sequence:
	GACAACCGGTCAAAACAACAATGCCAACTTTGCCTGGACT
S671A	Wild-type primer sequence:	TCT→GCT	T→A AluI disappears
	CCTTCAACCAGTCAAAGCTGAACTCTTTCATCACGCAATACA
	Mutant primer sequence:
	CCTTCAACCAGTCAAAGCAGAACGCTTTCATCACGCAATACA
T252A	Wild-type primer sequence:	ACC→GCC	C→G NaeI appears
	ACCCGAACCTGGGCCCTGCCCACCTACAACAACCACCTCTAC
	Mutant primer sequence:
	ACCCGAACCTGGGCCCTGCCGGCCTACAACAACCACCTCTAC
K137R	Wild-type primer sequence: GGTTGAGGAAGGCGCTAAGACGGCTCCTGG	AAG→AGG	C→G HhaI disappears
	Mutant primer sequence:
	GGTTGAGGAAGGGGCTAGGACGGCTCCTGG