Table 5.

Neutralization Antibody Formation Against Wild-Type or Mutant AAV8 Vectors

S. No.	Groups	Reciprocal NAb titer
1	Mock	0
2	WT-AAV8	320
3	S279A	320
4	S501A	640
5	S671A	320
6	K137R	160
7	Anti-AAV8 rabbit control serum	20480

Pooled serum samples from WT-AAV8 or S/K-AAV8 mutant injected mice (n=4 per group) 4 weeks after vector administration was studied. Heat-inactivated serum samples were assayed for the neutralizing antibody (NAb) titers as described in Materials and Methods. The NAb titer is reported as the highest plasma dilution that inhibited AAV transduction of Huh7 cells by 50% or more compared with that of the naive serum control. Limit of detection of the assay was 1/5 dilution.