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Time from luciferin injection to peak bioluminescence. Animals were anesthetized and injected with luciferin through either an SC or IP route, and images were collected beginning at 1 min after luciferin injection and imaging continued for 61 min. Bioluminescence is plotted against time. Peak and time-to-peak values were extrapolated from the plots. Error bars represent SE. IP, intraperitoneal; SC, subcutaneous.
