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. Author manuscript; available in PMC: 2013 Aug 2.
Published in final edited form as: Surgery. 2012 Oct 24;152(6):1068–1077. doi: 10.1016/j.surg.2012.08.021

Fig 5.

Fig 5

Assessment of apoptosis in representative pancreata from Pdx1-Cre:Men1 floxed/floxed conditional knockout mice following monthly subcutaneous (SC) injections of PBS or pasireotide (SOM230) (160mg/Kg/month [64mg/ml]) for four months by Terminal deoxynucleotidyl transferase dUTP Nick End-Labeling (TUNEL) assay. (A) Immunofluorescent staining of representative pancreas with insulinoma from the control group receiving monthly SC injections of PBS at the end of four months. Nuclei were visualized via DAPI staining (blue), whereas apoptotic foci were identified by terminal deoxynucleotidyl transferase-mediated addition of fluorescein-dUTP (green). (B) Immunofluorescent staining of representative pancreas with insulinoma from the treatment group receiving monthly SC injections of SOM230 at the end of four months. (C) Quantification was achieved by counting the apoptotic fluorescent cells under a fluorescent microscope (expressed as the percentage of total cells ± standard deviation [SD]).