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. Author manuscript; available in PMC: 2014 Jan 29.
Published in final edited form as: Biochemistry. 2013 Jan 14;52(4):627–639. doi: 10.1021/bi301609e

Table 2. Characterization of DamX SPOR domain mutants (fused to GFP).

Map of GFP-DamX constructsa ΔdamX backgroundb ΔdamXdedD backgrounde
Septal Localization (%)c DC resistanced Rescue divisionf Inhibit divisionf
graphic file with name nihms435113t1.jpg GFP-DamX(WT) 71 ±4 +++ +++ +++
graphic file with name nihms435113t2.jpg GFP 0 - - -
graphic file with name nihms435113t3.jpg GFP-DamX(Q351K) 8±1 - - -
graphic file with name nihms435113t3.jpg GFP-DamX(S354K) 26 ±6 +/- - -
graphic file with name nihms435113t3.jpg GFP-DamX(W416L) 40 ±7 + - -
graphic file with name nihms435113t4.jpg GFP-DamX(A350-428) 2±1 - - -
graphic file with name nihms435113t5.jpg GFP-DamX(A346-428) 2±1 - - -
a

For clarity, domains not drawn to scale. Cyto, cytoplasmic domain; TM, transmembrane helix; Linker, putative unstructured linker region; SPOR, SPOR domain.

b

The following derivatives of EC1910 were used: EC2882, EC2884, EC2886, EC2888, EC2965, EC2966, and EC2967.

c

The percentage of cells in the population judged to have a fluorescent band at the division site. Values are the average ± S.D. of 2-6 separate assays, with at least 100 cells scored each time.

d

Growth in the presence of 0.1 % deoxycholate. See supplemental figure S7 for the original data.

e

The following derivatives of EC1926 were used: EC2312, EC2313, EC2314, EC2315, EC2316, EC2785, and EC2786.

f

Cell length during growth in the presence of various concentrations of IPTG. See supplemental figure S8 for the data.