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. 2013 Aug 2;8(8):e70804. doi: 10.1371/journal.pone.0070804

Figure 1. Expression of CA-Lyn promotes the survival of nutrient-deprived U87 human GBM cells.

Figure 1

U87 human GBM cells expressing CA-Lyn, DN-Lyn or LV control were plated in DMEM with L-glutamine and 10% FBS. After 12 h, nutrient deprivation was induced by replacing the medium with L-glutamine- and FBS-free DMEM with 1% BSA. A, After 48 h of nutrient deprivation, whole cell lysates were western blotted with the indicated antibodies. B-D, After 7 days of nutrient deprivation, cell viability was determined by trypan blue exclusion (B–D) and apoptosis by FACs analysis of Annexin-V-labeled cells (E), blotting for cleaved caspase-7 (F), or FACS analysis for Annexin-V and propidium-iodide-labeled cells (G). B–E, and G, Conditions were assayed in replicas of 3 or 4, and the data analyzed and plotted as the mean±SEM. D & E, Statistical analysis using one-way ANOVA. G, Statistical analysis using t-test.