Figure 4. Expression of CA-Lyn increases levels of pAMPK in nutrient-deprived GBM cells.

A-D, U87 GBM cells expressing CA-Lyn, DN-Lyn or the LV control were plated and subjected to nutrient deprivation as described in Figure 1A. A-B, 15 h prior to detergent lysis (day 5), cells were treated with vehicle, chloroquine (CQ; 10 µM) (A), or 3-methylamine (3-MA; 5 µM), and then whole cell lysates western blotted with the indicated antibodies. C & D, Cells were detergent lysed after five days of nutrient deprivation and blotted with the indicated antibodies. The densitometric readings for the LC3B-II band were normalized to GAPDH, the pS6 band normalized to total S6, and the pAMPK band normalized to total AMPK.