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. 2013 Aug 2;8(8):e70092. doi: 10.1371/journal.pone.0070092

Figure 2. Immunoblot serotyping of K. pneumoniae NTUH-K1790N, Ca0507, Ca0421, and C1975.

Figure 2

Rabbit anti-C1975 antiserum was used as the first antibody (1∶5000) and goat anti-rabbit IgG-HRP as the second antibody (1∶10 000). (A) The slot blot showing anti-C1975 antiserum reacts to the extracted capsular polysaccharides from strains NTUH-K1790N, C1975, Ca0421 and Ca0507. Ca0507Δorf8 presents the isogenic mutant with a deletion of gene orf8 in the cps. (B) The slot blot for the extracted capsular polysaccharides from 77 K serotype reference strains and a new type A1517. Sample1–9: K31 to K39; 10: C1975 as control. Negative reactions were observed in other reference strains and A1517 (data not shown).