Fig. 3.

ATM depletion and hypoxia markers accumulation. (A, left) Upregulation by hypoxia (1% O₂) of ATM and other family members in proliferating ihNSCs as detected by western blot. (A, right) HIF-1α and CAIX protein levels in proliferating ihNSCs in relation to hypoxia and ATM deficiency. The graphs refers to the densitometry quantification of the western blot bands (**P < 0.01; *P < 0.05). (B) Hypoxic markers accumulation during differentiation. Cells grown in 1% O₂ were collected at various days of differentiation and the expression of HIF-1α and CAIX was examined by western blot. Protein loading per lane was verified with anti-vinculin or anti-β-Actin antibodies.