Fig. 4.

ATM depletion, hypoxia and effects on differentiation. ihNSCs at D17 of differentiation in the indicated O₂ concentrations were IF-labelled with antibodies specific for neurons (β-Tubulin III), GABAergic neurons (GABA) and oligodendrocytes (GalC). Nuclei were counterstained with DAPI and cells scored by fluorescence microscopy. Results in each graph were obtained from the analysis of three independent experiments, and significant differences between shCon and shATM are indicated (**P < 0.01; *P < 0.05; n.s.: not significant). The representative IF photos refer to shCon at D17 of differentiation in 5% O₂. (B) ihNSCs were induced to differentiate on glass coverslips in medium containing 1 mM NAC or 1 mM BSO, a glutathione-depleting and oxidative stress-inducing agent, and then IF-labeled with antibodies specific for oligodendrocytes (GalC) (**P < 0.01; *P < 0.05).