Figure 5.
Kinetic Modeling of Ubiquitination without DUBs versus with DUBs
(A) Normalized ubiquitination profiles of reactions performed in liposomes (green) versus microsomes (orange).
(B) Kinetic model of substrate ubiquitination, substrate dissociation, and deubiquitination. See Figure S6C for additional details.
(C) Output of the kinetic model at 20 s using the indicated parameters without (green) or with (orange) DUBs. The relative amounts of the individual ubiquitinated species are plotted.
(D) Relationship between polyubiquitination (defined as four or more ubiquitins), koff, and DUB activity predicted by the model analyzed at 20 s.
See also Figure S6.