Figure 1.
Rate of Accumulation of TF Binding Occupancy Differences between Closely Related Mammals within One Order
(A) To assess the rate at which TF binding differences accumulate, we identified and compared the global in vivo binding of FOXA1, CEBPA, and HNF4A in livers of six closely related rodents ranging in evolutionary separation from 1 to 20 MY. Examples of both shared and species-specific TF binding locations are indicated at representative loci.
(B) The fraction of FOXA1 binding found at homologous locations when comparing C57BL/6J and other rodent species (y axis) is plotted against the evolutionary distance between species in millions of years (x axis).
(C) FOXA1 binding intensities were compared across the entire genome within and between mouse species. TF binding profiles between individuals within the same species (C57BL/6J) showed a high correlation (green inset, R2 = 0.77), which decreased with increasing evolutionary distance.
See also Figures S1 and S2.