Figure 5.

Western blot analysis of epidermal keratins. (A) The loss of K10 expression in K10^−/− epidermis was confirmed by Western blotting, and its reduced expression in heterozygote skin (+/–) was shown. Note a remarkable decrease in K1 in K10^−/− pups and a slight decrease in the skin of heterozygotes. (B) The amount of K5 remained unaltered, whereas its partner K14 was slightly increased. The amount of the third basal keratin, K15, was unaltered. Equal loading was verified by quantitative comparison of Coomassie blue staining. Lanes 1–3, Coomassie blue–stained polyvinylidene difluoride membrane; lanes 4–6, corresponding Western blot; M, marker; dots from top to bottom, 66.4, 55.6, and 42.7 kDa.