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. Author manuscript; available in PMC: 2014 Aug 15.
Published in final edited form as: Phys Chem Chem Phys. 2013 Aug 14;15(30):12516–12524. doi: 10.1039/c3cp50880c

Figure 1.

Figure 1

a) Experimental setup for in situ SFG measurements. A CaF2 window is coated with a 25 nm gold layer and then decorated with a DoDT SAM. b) SFG C–H spectra collected in situ through the backside of the window. For the LKα14 spectrum a deuterated DoDT SAM was used as substrate to avoid spectral confusion with the peptide CH modes. The peptide and SAM peaks have a phase difference of >π/2 showing the CH3 orientations of these two species have a predominately opposite orientation. c) Schematic showing the orientation of the leucine isopropanol groups binding to the DoDT methyl groups.