Skip to main content
. Author manuscript; available in PMC: 2013 Aug 3.
Published in final edited form as: Nat Methods. 2011 Feb 20;8(4):335–340. doi: 10.1038/nmeth.1574

Figure 2.

Figure 2

Interlacing and timesharing of optical trap and fluorescence excitation lasers. Two optical traps are created in sequence during time intervals A and B via the trap AOM switching between two trap laser intensities and deflection angles (traps in intervals A and B were set to different intensities for clarity in the figure). Trap data acquisition occurs at time points indicated by ‘x’ and ‘+’ for traps 1 and 2 respectively. The fluorescence excitation laser is only ON during time interval C while the trap laser is OFF. There are 625 ns delays (hatched intervals) between switching optical traps OFF (ON) and fluorescence excitation ON (OFF). Fluorescence is only collected during time interval C. Laser intensities in the plots were measured by photodetectors and recorded by a digital oscilloscope.