Figure-2. Significance of let-7 down-regulation in malignant-GCTs.
A) Sylamer landscape plots for SCR words corresponding to the common seed of the nine let-7 microRNA family members in the combined analysis of pediatric (set-1) and adult (set-2) malignant-GCTs. Log10-transformed p-values for each SCR word are plotted on the y-axis, against the ranked gene list on the x-axis. A negative y-axis deflection on the right-hand side of the plot signifies SCR enrichment in up-regulated genes. The left-hand plot shows data for the hexamer complementary to the core 2-7nt component of the common seed-region, the central plot the two heptamers (1-7nt; 2-8nt), and the right-hand plot the octamer (1-8nt). The single summed significance score and p-value for all four SCR words is shown. B) Levels of the six selected let-7 mRNA targets in sample set-3, determined by qRT-PCR. Error-bars=SEM. C) Correlations between each mRNA and let-7e in set-3. P-values were determined by linear-regression.