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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Cancer Res. 2013 Jun 17;73(15):4872–4884. doi: 10.1158/0008-5472.CAN-12-2085

Figure-7. Luciferase assay confirmation of let-7 targets in malignant-GCT cells.

Figure-7

Luciferase assay data at d2 for 2102Ep cells transfected with a reporter containing the full-length 3′UTR for LIN28 (red), MYCN (blue) or AURKB (orange). Cells were also transfected with either let-7e or let-7e-mutant (let-7e-mut). Luminescence values were normalized to cells treated with non-targeting oligonucleotides (NT2), then referenced to cells containing a no 3′UTR control reporter and treated with let-7e/let-7e-mutant, as appropriate. Error-bars=SEM. All correlation p-values were determined by linear-regression. *=p<0.05;**=p<0.005;***=p≤0.0001.