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. Author manuscript; available in PMC: 2013 Aug 3.
Published in final edited form as: Nat Immunol. 2008 Aug 31;9(10):1157–1164. doi: 10.1038/ni.1645

Figure 4.

Figure 4

Rapamycin does not affect TLR9 expression or interaction with CpG-A. (a) Flow cytometry of isolated spleen pDCs stimulated with CpG-A with or without rapamycin pretreatment, then stained for intracellular TLR9. (b) Deconvolution microscopy of RAW cells expressing YFP-tagged TLR9 (green), pretreated with vehicle (left) or rapamycin (right) and then incubated for 90 min with indodicarbocyanine-tagged CpG-A–DOTAP (red; 5 μg/ml). Two images were obtained for each condition. Data are representative of two independent experiments.