Figure 1.

Deregulated expression of c-Myc elicits apoptosis induction by cisplatin. Exponentially growing Rat-1/MycER^TM (myc) or Rat-1/ΔMycER^TM (Δmyc) cells were exposed to OHT for 16 h or left untreated (lanes C in panel A, middle panels in B anc C) and then exposed to cisplatin (cDDP) for 3 (C), or 6 h (A, B) at the indicated concentration of 0, 10, 25, or 50 μM, or for 1 h at a concentration of 25 μM (D). Lanes M contain DNA markers. Internucleosomal DNA degradation (A), condensed and fragmented nuclei (B), cell blebbing (C), and clonogenic survival (D) were evaluated as described in MATERIALS AND METHODS. The means and SDs were calculated from at least three separate experiments.