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. Author manuscript; available in PMC: 2015 Jan 1.
Published in final edited form as: Transfusion. 2013 Apr 29;54(1):179–189. doi: 10.1111/trf.12217

Figure 5. Circulating anti-KEL glycoprotein Igs bind to transfused KEL2 RBCs and are associated with KEL2 RBC clearance.

Figure 5

KEL2 and C57BL/6 RBCs were labeled with DiI and DiO, respectively, prior to transfusion. RBC bound IgM (A) and IgG (B) were evaluated serially post-transfusion on DiI positive KEL RBCs; shaded histograms are control antigen negative cells. Post-transfusion survival and recovery of KEL2 RBCs was determined by comparing a ratio of DiI KEL to DiO C57BL/6 RBCs (C). These studies were completed KEL2 and wild type C57BL/6 recipients (D); similar studies were also completed following a 2nd KEL2 transfusion in recipients initially transfused with or without poly (I:C) (E). Error bars represent standard deviation and results represent at least 3 independent experiments with 3–5 mice/group.