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Limit of detection of soman-labeled human albumin on a Western blot. Lanes were loaded in duplicate with 0.5 to 0.005 μg of soman-labeled albumin. The blot was hybridized with fourth bleed antiserum diluted 1:2000. Samples were detected via chemiluminescence using horseradish peroxidase linked to anti-rabbit IgG. The limit of detection was 0.01 μg of soman-labeled human albumin.
