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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Nature. 2013 Jun 30;500(7460):107–110. doi: 10.1038/nature12302

FIGURE 1. Chemical differences between uridine and pseudouride and experimental set-up.

FIGURE 1

(a) Uridine (U, 1-ß-D-ribofuranosyluracil) and pseudouridine (Ψ, 5-ß-D-ribofuranosyluracil). (b) Top, diagram of the three synthetic mRNA constructs designed for the in vitro nonsense suppression experiment in bacteria. Bottom, anti-His and anti-Flag immunoblot analysis of the in-vitro translation assays in E. coli. (c) The tRNASer anticodon stem loop (ASL) and mRNA used in this study.