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. 2004 Jan 2;32(1):169–178. doi: 10.1093/nar/gkg925

Figure 4.

Figure 4

Biochemical analysis of the Rad51B–Rad51C interaction. (A) Insect cells were co-infected with baculovirus expressing Glu-tagged Rad51C alone (lane 4) or with full-length His–Rad51B (lane 1), His–Rad51B1–75 (lane2) or His–Rad51B76–350 (lane 3) as indicated with + or – symbols at the top of (I). (I) Western analysis with anti-His monoclonal antibody of co-infected insect cells detecting the His-tagged full-length Rad51B and Rad51B fragments. (II) Western analysis with anti-Rad51C monoclonal antibody detecting Glu–Rad51C. (III) Immunoprecipitation of His–Rad51B proteins with the anti-His antibody subsequently blotted with the anti-Rad51C monoclonal antibody. (B) Insect cells were co-infected with baculovirus expressing Glu-tagged Rad51B alone (lane 4) or with full-length His–Rad51C (lane 1), His–Rad51C1–79 (lane 2) or His–Rad51C70–376 (lane 3) as indicated with + or – symbols at the top of (I). (I) Western analysis using an anti-Rad51B polyclonal antibody detecting expression of Glu-tagged Rad51B in all four lanes. Rad51B is most highly expressed alone in lane 4. (II) Western analysis of the same lysates with anti-His monoclonal antibody to detect His–Rad51C (lane 1), His–Rad51C1–79 (lane 2) or His–Rad51C70–376 (lane 3). (III) Immuno precipitation of samples with anti-His antibody followed by western blotting with anti-Rad51B polyclonal antibody.