Table 2. Hydrodynamic parameters of DNA nanoparticles formed in the presence of different polyamines.
Polyamine | Polyamine concentration used for nanoparticle formation (µM) | Diffusion coefficient (cm2/s) | Hydrodynamic radius (nm)a |
---|---|---|---|
3-4-3 (Spermine) | 25 | 2.4 × 10–8 | 94.6 ± 1.6 |
3-3-3 | 25 | 2.5 × 10–8 | 91.8 ± 5.3 |
3-3-3-3 | 5 | 2.8 × 10–8 | 80 ± 10.4 |
3-3-3-3-3 | 2 | 3.4 × 10–8 | 67 ± 6.4 |
3-4-3-4-3 | 2 | 4.5 × 10–8 | 51 ± 1.6 |
aAll measurements were made in 10 mM Na cacodylate buffer with the indicated concentrations of polyamines. Data given are mean ± S.E.M. of 3–5 separate measurements.