Table 1.
Strains and plasmids | Characteristicsa | Source/references |
---|---|---|
Strains | ||
E. coli DH10B | Host strain for cloning commercial | Invitrogen |
L. plantarum WCFS1* | Host strain, plasmid-free, silage isolate | Kleerebezem et al. (2003) [25] |
Plasmid | ||
pLp_0373sAmyA | p256/pUC(pGEM)ori; PsppA; Lp_0373; amyA; Emr | Mathiesen et al. (2009) [24] |
pLp_3050sAmyA | p256/pUC(pGEM)ori; PsppA; Lp_3050; amyA; Emr | Mathiesen et al. (2009) [24] |
pLB36 | pET 32a oxdC; Amr | Just et al. (2004) [26] |
pSip-OxdC | p256/pUC(pGEM)ori; PsppA; oxdC; Emr | Kolandaswamy et al. (2009) [23] |
pLp_0373sOxdC | p256/pUC(pGEM)ori; PsppA; spLp_0373 fused to the oxdC; Emr | This work |
pLp_3050sOxdC | p256/pUC(pGEM)ori; PsppA; spLp_3050fused to the oxdC; Emr | This work |
aFor strains, genotypic and phenotypic characteristics are given; for plasmid, plasmid and cloned-cassette characteristics are given; Emr, Amr: resistance to erythromycin and ampicillin, respectively.
*L. plantarum WCFS1 is a single colony isolate of strain NCIM8826 (Kleerebezem et al., 2003 [25]).