Table 3.
Oxalate decarboxylase (OxdC) activity in concentrated culture supernatant and cell lysate of various recombinant L. plantarum WCFS1 strainsa.
| Name of strain (plasmid) | Protein localization | OxdC (U/mg protein) activity in culture supernatant at OD600 = 1.7 | OxdC (U/mg protein) activity in cell fraction at OD600 = 1.7 | Secretion efficiency* (%) |
|---|---|---|---|---|
| WCFS1 (Control) | NP | NA | NA | ND |
| WCFS1 (pSIP-OxdC) | Intracellular | NA | 2.8 ± 0.2 | ND |
| WCFS1 (pLp_0373sOxdC) | Extracellular | 0.05 ± 0.01 | 0.18 ± 0.07 | 22 |
| WCFS1 (pLp_3050sOxdC) | Extracellular | 0.02 ± 0.01 | 0.21 ± 0.07 | 9 |
aData are mean of three independent experiments, NP: no production, NA: no activity, and ND: not determined, and * secretion efficiency were determined at OD600 = 1.7 between the culture supernatant and cell fraction of respective strains.