Figure 8.

Pulse–chase analysis of yeast telomerase. (A) Yeast telomerase was subjected to pulse–chase analysis using the TEL15(m4,5) primer as described in Materials and Methods. The duration of the pulse and chase reactions and the presence or absence of the TG40 competitor primer are indicated at the top. Products due to TEL15(m4,5) and TG40 are indicated by brackets at the right. First and second round products for TEL15(m4,5) are indicated by brackets at the left (‘P + 1 to P + 7’ and ‘>P + 7’, respectively). (B) The ratios of the second round products (>P + 7) to the first round products (P + 1 to P + 7) were determined for assays 1–4 shown in part (A) and plotted.